Hansatech FMS-300, Pulse-modulated chlorophyll fluorometer
Pulse-modulated chlorophyll fluorometer for teaching & research
Overview
FMS-300 is a state-of-the-art, Pulse Amplitude Modulated (PAM) chlorophyll fluorometer combining the usability of a teaching system with the power and functionality to provide high-level research-grade data. A wide range of accessory components allow experiments to be conducted on many different sample types.
Newcomers to the technique are quickly able to acquire and analyse data, making FMS-300 an ideal system for teaching chlorophyll fluorescence. Yet it is also a highly capable research instrument offering flexibility, functionality, and data acquisition of exceptional quality. Extensive features and capabilities allow the system to be used to demonstrate complex concepts, conduct experiments, collect data, analyse results, and facilitate collaborative learning or research projects.
Primarily a laboratory-based system, FMS-300 can extend to greenhouse and field applications when coupled with an appropriate portable power source. The enviable signal quality is achieved via ultra-short measuring pulses with a standard frequency of 10 Hz (up to 100 kHz during fast fluorescence capture).
At just 400 ns per pulse, FMS-300 can emit high-intensity measuring pulses with user-defined average intensities up to 0.1 μmol m-2 s-1.
This combination of high-intensity and ultra-short pulse width produces a strong fluorescence signal with no requirement for signal damping or data averaging. The user is presented with 100% raw instrument data.
Key Features
- Versatile functionality: Suitable for both teaching and research applications. FMS-300 can demonstrate complex concepts, conduct experiments, collect data, analyse results, and facilitate collaborative learning
or research projects. - Fast & slow fluorescence data: Presents fast and slow fluorescence data with equal emphasis. Fast fluorescence kinetics are captured during every saturating pulse for both dark and light-adapted sample states,
presenting opportunities for novel research. - Comprehensive parameter display: Parameter sets relevant to the selected routine are calculated for each saturating pulse. For dark-adapted samples, OJIP parameters in addition to parameters such as Fv/Fm are calculated.
Light-adapted parameters including ETR, ΦPSII, Lake and Puddle models for non-photochemical quenching, and both a calculated Fo’ and Fo’ measured under far-red light are calculated where appropriate. - Developed for high performance: measuring pulse width of 400 ns from a dedicated LED allows greater intensities to be implemented yielding a strong, low-noise signal. Measuring pulse intensity can be defined
between *0.001 - 0.1 μmol m-2 s-1. Actinic and saturating pulse intensities of up to 3,000 and **20,000 μmol m-2 s-1 respectively can be delivered at the sample surface. - Streamlined data capture: FluoroControl software provides 6 pre-set experimental routines, which can be further configured, if required. This enables rapid learning and efficient, hassle-free data acquisition.
- Intuitive software with simplified control set: Developed from the ground up with simplicity in mind. A user-friendly interface with minimal learning curve allows easy navigation of the different functions and options.
- Data visualisation & analysis:The measuring LED performance means that 100% raw data is presented with no requirement to damp or average. Robust data visualisation, analysis and export tools allow users to interpret
and present findings effectively. Valuable for both teaching complex concepts and conducting in-depth analysis in research applications. - Measuring light & actinic/saturating LED colours: 4 variants with different pairings of LED colour. Supplied with either blue/blue (455 nm), red/red (624 nm), blue/white or red/white measuring/actinic and saturating LEDs. All variants contain a
far-red LED (730 nm) for determination of Fo’ during the quenching analysis routine.
* Depending on optical accessory in use. Up to 0.1 μmol m-2 s-1 when using dark-adaptation or PTL-100 leafclips. Up
to 0.41 μmol m-2 s-1 when using FMS/LG3 tapered light guide.
** Depending on optical accessory in use. Up to 60,000 μmol m-2 s-1 when using FMS/LG2 tapered light guide and up
to 90,000 μmol m-2 s-1 when using FMS/LG3 tapered light guide.
Tilleggsinformasjon
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Spesifikasjoner
Control unit
All light intensity specifications refer to incident illumination at the sample surface.
| Measuring LED: |
Pulse width: 400 nanoseconds.
Pulse frequency: Slow kinetics: 10 Hz.
Fast kinetics: Semi-log frequencies from 10 Hz-100 kHz.
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| Pulse intensity: | Up to 0.1 µmol m-2 s-1 in FMS-300-B/BW. Up to 0.058 µmol m-2 s-1 in FMS-300-R/RW. Adjustable in 0.001 µmol m-2 s-1 increments. With light guide, FMS/LG2: >0.25 µmol m-2 s-1. With light guide, FMS/LG3: >0.4 µmol m-2 s-1. |
| Measuring LED colour: | FMS-300-B/BW: Blue 455 nm (FWHM 27 nm). FMS-300-R/RW: Red 624 nm (FWHM 18 nm). |
| Actinic/saturating pulse LED colour: | FMS-300-B/BW: Blue 455 nm (FWHM 27 nm). FMS-300-R/RW: Red 624 nm (FWHM 18 nm). |
| Max. actinic intensity: | Up to 3,000 µmol m-2 s-1. Adjustable in 1 µmol m-2 s-1 increments. With light guide, FMS/LG2: >8,000 µmol m-2 s-1. With light guide, FMS/LG3: >12,000 µmol m-2 s-1. |
| Max. saturating pulse intensity: | >20,000 µmol m-2 s-1. Adjustable in 1 µmol m-2 s-1 increments. With light guide, FMS/LG2: Up to 60,000 µmol m-2 s-1. With light guide, FMS/LG3: Up to 90,000 µmol m-2 s-1. |
| Far-red LED: | 730 nm (FWHM 20 nm). |
| Far-red max. intensity: | Up to 20 µmol m-2 s-1. With light guide, FMS/LG2: up to 60 µmol m-2 s-1. With light guide, FMS/LG3: up to 90 µmol m-2 s-1. |
| Detector: | PIN photodiode, rapid peak-pulse tracking. |
| Detection range: | FMS-300-B: >645 nm. FMS-300-R/BW/RW: >680 nm. |
| Electronics: |
Dual processors: ARM 32-bit microcontroller running real-time operating system.
PIC 8-bit microcontroller for dedicated measuring LED sampling.
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| Fibre-optic cable: | Length 1 m, statistically randomised fibre bundles. 7mm optical diameter at sample end. |
| Connections: | Optical connection for fibre-optic cable. 12 V DC socket. USB-C socket. MiniDIN socket for PTL-100. |
| Enclosure: | Shielded aluminium enclosure. |
| Dimensions: | 150 (l) x 150 (w) x 85 mm (d). Weight 770g. |
| Communications: | USB 2.0. Cable type A – C. |
| Operating conditions: | 0°C-40°C. Non-condensing humidity. |
| Power supply: | 12 V DC mains power. |
| Power consumption: | 5.6-7.2 W. Max. 50 W. |
| Display: | 4 line x 20 character blue LCD display. |
Leafclips & accessories
| FMS/DLC: | Nylon 3D printed, 7 mm sample aperture, sliding shutter blade, 60° angle between fibre-optic & sample. |
| PTL-100: | Nylon 3D printed, leaf temp. & PAR (400-700 nm) sensor. Electrical connection to FMS-300. Open faced, 60° angle between fibre-optic & sample. |
| Manfrotto support arm: | Mini Variable Friction Arm. 0.44kg, length: 12-24 cm, max. payload: 3 kg. |
| Manfrotto clamp: | Nano Clamp. 0.097 kg. clamp range 13-35mm, max. payload: 4 kg. |
| Transport case: | 464 (l) x 366 (w) x 176 (d) mm. Weight: ~3 kg. |
Software requirements
| Operating System: | Windows 10 or newer. |
System components
- FMS-300 control unit.
Options for LED colours to be specified at point of order:- FMS-300-B: blue measuring, actinic and saturating LED
- FMS-300-R: red measuring, actinic and saturating LED
- FMS-300-BW: blue measuring with white actinic and saturating LED
- FMS-300-RW: red measuring with white actinic and saturating LED
- FMS/SFO fibre optic cable
- PTL-100 PAR temperature leafclip
- FMS/DLC pack of 10 dark-adaptation leafclips
- Fibre optic cable support arm and clamp
- Power supply
- USB connection cable
- Protective hard case
Accessories:
| FMS/LC Liquid Sample Chamber:
Allows measurement of liquid-phase samples.
Samples may be dark-adapted prior to measurement.
Can be used with or without magnetic stirrers for sample mixing during measurement.
Multiple chambers increase sample throughput potential in screening protocols.
Compatible with both FMS-300 and FMS 2+ chlorophyll fluorometers.
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| FMS/DLC Dark-adaption leafclips:
Additional dark adaption leafclips for FMS modulated fluorometers.
Allows samples to be dark adapted for measurements of Fv/Fm.
3D printed nylon construction with stainless steel shutter plate.
10 leafclips per pack.
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FMS/LGx Light Guide:
Allows measurements of samples not suitable for use with conventional leafclips
Can be submerged in liquid for aquatic and marine applications
Optical grade borosilicate glass construction for maximum transmission of light and fluorescence signal
Compatible with both FMS-300 and FMS 2+ chlorophyll fluorometers
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